Overview

Fred Hutchinson Cancer Center is an independent, nonprofit organization providing adult cancer treatment and groundbreaking research focused on cancer and infectious diseases. Based in Seattle, Fred Hutch is the only National Cancer Institute-designated cancer center in Washington.

With a track record of global leadership in bone marrow transplantation, HIV/AIDS prevention, immunotherapy and COVID-19 vaccines, Fred Hutch has earned a reputation as one of the world's leading cancer, infectious disease and biomedical research centers. Fred Hutch operates eight clinical care sites that provide medical oncology, infusion, radiation, proton therapy and related services, and network affiliations with hospitals in five states. Together, our fully integrated research and clinical care teams seek to discover new cures to the world's deadliest diseases and make life beyond cancer a reality.

At Fred Hutch we value collaboration, compassion, determination, excellence, innovation, integrity and respect. These values are grounded in and expressed through the principles of diversity, equity and inclusion. Our mission is directly tied to the humanity, dignity and inherent value of each employee, patient, community member and supporter. Our commitment to learning across our differences and similarities make us stronger. We seek employees who bring different and innovative ways of seeing the world and solving problems. Fred Hutch is in pursuit of becoming an anti-racist organization. We are committed to ensuring that all candidates hired share our commitment to diversity, anti-racism and inclusion.

The Li research group (https://research.fredhutch.org/haolong-li/en.html) at the Fred Hutch Cancer Center has an opening for a Research Technician II for a scientist interested in contributing to efforts to develop new treatments for advanced cancer. The Li group is embedded within a highly collaborative research environment in the Prostate cancer program, which is comprised of scientists with a diverse range of skills and expertise. The successful applicant will apply functional genomics, including CRISPR screen approaches (CRISPRi/a and single-cell Perturb-seq), to systematically identify novel targets and understand resistance mechanisms. There is ample opportunity to learn new skills and contribute to research projects.

Our research team is looking for an outstanding Research Technician with a strong interest in cancer biology, immunology, and cutting-edge CRISPR technologies. The technician will perform both standard and investigative laboratory work. Responsibilities include molecular biology techniques, cell culturing, flow cytometry, CRISPR gene editing, and tumor xenograft studies in mice. Additional duties involve managing mouse colonies, conducting pharmacologic experiments, and assisting with lab maintenance and data analysis.

Responsibilities

Key Responsibilities (skills can be taught):

• Perform molecular biology experiments, including cloning, DNA/RNA isolation, protein expression analysis, and preparing next-generation sequencing libraries.
• Culture cancer cell lines,immune effector cells, and cancer organoids. Conduct flow cytometry and immunofluorescence.
• Execute knock-down experiments using shRNA and CRISPR-based techniques. Perform gene transfer studies using lentiviral infection.
• Perform mouse procedures such as tumor xenografting, IV/IC/IP injections, drug/reagent administration, and imaging (IVIS and ultrasound).
• Conduct xenograft studies and pharmacologic experiments in mice, including using immunotherapies like CAR immune effector cells and performing immunohistochemistry.
• Manage mouse colonies, including breeding, weaning, tagging, genotyping, and recording data for tracking.
• Assist with lab maintenance duties, stocking reagents, attending lab meetings, and presenting results.
• Read relevant scientific literature.
• Assist with manuscript/grant preparation and optional computational data analysis.

Qualifications

MINUMUM QUALIFICATIONS

• Bachelor's degree in a related scientific field.
• At least two years of full-time, post-bachelor's laboratory experience.

PREFERRED QUALIFICATIONS

• Experience with mouse experiments such as xenografts and/or husbandry.
• Experience with molecular biology and biochemistry techniques, cell culture, gene transfer studies, and/or managing a mouse colony.
• Ability to work well as part of a team with outstanding written and oral communication skills.
• Self-motivated, fast learner, critical thinker, superior organization, and enthusiastic about science.
• Independent research experience.
• Familiarity with R or other programming languages is an asset.

We will consider applicants from diverse backgrounds and with different levels of experience. We will also base our evaluation on your specific interest and motivation for this position. Accordingly, in your application please include a cover letter that describes your interest and motivation related to Prostate cancer research along with your CV and contact information for at least two references.

A statement describing your commitment and contributions toward greater diversity, equity, inclusion, and antiracism in your career or that will be made through your work at Fred Hutch is requested of all finalists.

The hourly pay range for this position is from $25.71 to $36.60 and pay offered will be based on experience and qualifications. Fred Hutchinson Cancer Center offers employees a comprehensive benefits package designed to enhance health, well-being, and financial security. Benefits include medical/vision, dental, flexible spending accounts, life, disability, retirement, family life support, employee assistance program, onsite health clinic, tuition reimbursement, paid vacation (12-22 days per year), paid sick leave (12-25 days per year), paid holidays (13 days per year), paid parental leave (up to 4 weeks), and partially paid sabbatical leave (up to 6 months).

Our Commitment to Diversity