Carmel, Indiana
Posted: 17-Jul-24
Location: Los Angeles, California
Salary: Open
Categories:
Internal Number: 140293414
The Division of Hematology Oncology is seeking a skilled StaffResearch Associate with experience in a BSL2 laboratory to perform tissueculture work; maintain different cell lines and human cells; performlarge-scale selection of different populations of cells; grow and purifyviral vectors for HIV and cancer (transfection, vector purification usingultracentrifugation and TFF, titration) and live virus; maintain log ofvectors, virus, and cells. You will perform functional assays (cytokine assays)on human HSC cells and cell lines. Process blood and tissue samples(mouse, human, primate) and sort for selected populations of cells. HIVinfection of cultured cells and human cells. Perform molecular biology:DNA and RNA extraction, molecular cloning, PCR and RT-PCR, overlap PCR. Designprimers and synthesize plasmids using Snapgene and Ape sequencingsoftware. Perform Western Blots. Maintain laboratory notebook in enough detailthat results can be reproduced. Plan experiments in conjunction withsupervisor but also must be able to analyze and interpret data and planexperiments independently. Work closely with other technicians in conductinglarge-scale experiments. Assist in training of undergraduates. Order, stock,and maintain inventory on laboratory supplies. Assist with handling ofmice. Perform animal handling and manipulations - various types of injections,retro orbital bleeds, biopsies, euthanizations, and sample harvesting(blood, various tissues). Assist with humanized mouse surgeries. Monitor mousehealth and cell engraftment for downstream applications.
Salaryoffers are determined based on various factors including, but not limited to,qualifications, experience, and equity. UCLA Health hourly salary range forthis position is $26.52- $31.63.
Required:
- Skill inusing BSL2+ laboratory practices and sterile techniques.
- Abilityto work with HIV in a biosafety cabinet. Ability to grow virus stocks.
- Skill inpreparing media and reagents used in tissue culture work.
- Skill inhandling human cells and cell lines (adherent and suspension). Skill incounting cells, freezing/thawing cells, storing cells, passaging cell lines,maintaining primary cells.
- Skill inperforming functional assays (cytokine assays) in human cells and cell lines.
- Skill inperforming vector transfection, transduction, and titration.
- Skill inflow cytometry staining of samples (cells, blood, tissue).
- Skill inflow cytometry acquisition and analysis: MACSQuant and Fortessa flow cytometeruse.
- Skill inperforming DNA/RNA extractions, viral RNA, PCR, RT-PCR, overlap PCR.
- Skill inmolecular cloning: restriction enzyme digestion, bacterial cultures, plasmidpreparation. Skill in gelelectrophoresis.
